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Image Search Results
Journal: Molecular Therapy. Nucleic Acids
Article Title: Distinct Expression Profiles and Novel Targets of MicroRNAs in Human Spermatogonia, Pachytene Spermatocytes, and Round Spermatids between OA Patients and NOA Patients
doi: 10.1016/j.omtn.2017.09.007
Figure Lengend Snippet: Morphological and Phenotypic Characterization of Freshly Isolated Human Spermatogonia, Pachytene Spermatocytes, and Round Spermatids (A–C) Phase-contrast microscope revealed the morphology of the freshly isolated human pachytene spermatocytes (A), spermatogonia (B), and round spermatids (C) of OA patients. (D–F) DIC microscope showed the morphological characteristics of the freshly isolated human pachytene spermatocytes (D), spermatogonia (E), and round spermatids (F) of OA patients. Scale bars, 20 μm (A–C) and 5 μm (D–F). (G) RT-PCR revealed the transcripts of GPR125 , RET , GFRA1 , THY1 , UCHL1 , MAGEA4 , and PLZF in the fleshly isolated spermatogonia, the expression of SYCP3 and SYCP1 in pachytene spermatocytes, and mRNA of TNP1 , TNP2 , PRM1 , PRM2 , and ACR in round spermatids. RNA without RT (RT-) but with PCR of GAPDH primers was utilized as negative controls, and GAPDH served as loading controls of total RNA.
Article Snippet: The primary antibodies included THY1 (Abcam, ab133350, 1:200), GFRA1 (Santa Cruz, sc-6156, 1:200), PLZF (Santa Cruz, sc-22839, 1:200), UCHL1 (Bio-Rad, MCA4750, 1:200), PNA (Life Technologies, L32458, 1:200), and
Techniques: Isolation, Microscopy, Reverse Transcription Polymerase Chain Reaction, Expressing
Journal: Molecular Therapy. Nucleic Acids
Article Title: Distinct Expression Profiles and Novel Targets of MicroRNAs in Human Spermatogonia, Pachytene Spermatocytes, and Round Spermatids between OA Patients and NOA Patients
doi: 10.1016/j.omtn.2017.09.007
Figure Lengend Snippet: Phenotypic Characterization of Freshly Isolated Human Spermatogonia, Pachytene Spermatocytes, and Round Spermatids (A–D) Immunocytochemistry revealed the expression of THY1 (A), GFRA1 (B), PLZF (C), and UCHL1 (D) in the freshly isolated human spermatogonia. Scale bars, 10 μm. (E) Meiotic chromatin spread by triple immunostaining displayed the co-expression of CREST, SYCP3, and MLH1 in the freshly isolated human pachytene spermatocytes. Scale bar, 5 μm. (F–I) Immunocytochemistry demonstrated the expression of PNA (F), PRM2 (G), and rabbit IgG (I) in the freshly isolated human round spermatids and rabbit IgG (H) in the freshly isolated human spermatogonia. Scale bars, 10 μm.
Article Snippet: The primary antibodies included THY1 (Abcam, ab133350, 1:200), GFRA1 (Santa Cruz, sc-6156, 1:200), PLZF (Santa Cruz, sc-22839, 1:200), UCHL1 (Bio-Rad, MCA4750, 1:200), PNA (Life Technologies, L32458, 1:200), and
Techniques: Isolation, Immunocytochemistry, Expressing, Triple Immunostaining
Journal: Molecular Therapy. Nucleic Acids
Article Title: Distinct Expression Profiles and Novel Targets of MicroRNAs in Human Spermatogonia, Pachytene Spermatocytes, and Round Spermatids between OA Patients and NOA Patients
doi: 10.1016/j.omtn.2017.09.007
Figure Lengend Snippet: The Sequences of Gene Primers Used for RT-PCR and Real-Time PCR
Article Snippet: The primary antibodies included THY1 (Abcam, ab133350, 1:200), GFRA1 (Santa Cruz, sc-6156, 1:200), PLZF (Santa Cruz, sc-22839, 1:200), UCHL1 (Bio-Rad, MCA4750, 1:200), PNA (Life Technologies, L32458, 1:200), and
Techniques: